| 引物名称： | pTrcHis Forward、pTrcHis Forward |
|---|---|
| 引物又名： | pTrcHis Forward引物、pTrcHis Forward引物、pTrcHis Forward测序引物、pTrcHis Forward测序引物、pTrcHis Forward通用引物、pTrcHis Forward通用引 物、pTrcHis Forward通用测序引物、pTrcHis Forward通用测序引物、pTrcHis Forward Primer、 pTrcHis Forward Primer、pTrcHis Forward Sequencing Primer、pTrcHis Forward Sequencing Primer、 |
| 引物序列: | GAGGTATATATTAATGTATCG (Invitrogen) (Invitrogen) |
| 引物说明: | 5' of MCS in pTrcHis vector, forward primer |
| 引物类型： | 测序引物，通用引物，通用测序引物 |
| 级别： | HPLC |
| 用途: | 测序，PCR |
| 产品目录号： | P1017 |
| 产品规格： | 2OD、 10OD |
| 保存条件： | 密封，2-25℃保存。 |
Commonly Used Primers
| CMV Forward |
CGCAAATGGGCGGTAGGCGTG (Invitrogen) Human CMV immediate early promoter, forward primer |
| LKO.1 5' |
GACTATCATATGCTTACCGT (Weinberg Lab) Human U6 promoter, forward primer |
| LucNrev |
CCTTATGCAGTTGCTCTCC 5' end of luciferase, reverse primer |
| M13 Reverse |
CAGGAAACAGCTATGAC In lacZ gene |
| MSCV |
CCCTTGAACCTCCTCGTTCGACC (BD Biosciences) Murine stem cell virus, forward primer |
| pBABE 5' |
CTTTATCCAGCCCTCAC (Weinberg Lab) Psi packaging signal, 5' of MCS in pBABE vectors, forward primer |
| pGEX 5' |
GGGCTGGCAAGCCACGTTTGGTG 3' end of glutathione-S-transferase, forward primer |
| SP6 |
ATTTAGGTGACACTATAG SP6 promoter, forward primer |
| T3 |
GCAATTAACCCTCACTAAAGG T3 promoter, forward primer |
| T7 |
TAATACGACTCACTATAGGG T7 promoter, forward primer |
Full Primer List
| 3'AOX1 |
GCAAATGGCATTCTGACATCC (Invitrogen) For Pichia vectors with AOX1 terminator, reverse primer |
| 5'AOX1 |
GACTGGTTCCAATTGACAAGC (Invitrogen) For Pichia vectors with AOX1 promoter, forward primer |
| 35S promoter |
CTATCCTTCGCAAGACCCTTC CaMV 35S promoter, forward primer |
| AC5 |
ACACAAAGCCGCTCCATCAG (Invitrogen) Drosophila Actin 5C promoter, forward primer |
| Alpha-factor |
TACTATTGCCAGCATTGCTGC (Invitrogen) Alpha factor signal sequence, forward primer |
| Amp-R |
ATAATACCGCGCCACATAGC 5' end of ampicillin resistance gene, reverse primer |
| AUG1 Forward |
CAATTTACATCTTTATTTATTAACG (Invitrogen) For Pichia vectors with AUG1 promoter, forward primer |
| AUG1 Reverse |
GAAGAGAAAAACATTAGTTGGC (Invitrogen) For Pichia vectors with AUG1 promoter, reverse primer |
| BGH Reverse |
TAGAAGGCACAGTCGAGG (Invitrogen) Bovine growth hormone terminator, reverse primer |
| Bglob-intron-F |
CTGGTCATCATCCTGCCTTT Rabbit beta-globin intron, forward primer |
| Bglob-intron-R |
TTTGCCCCCTCCATATAACA Rabbit beta-globin intron, reverse primer |
| Bglob-pA-R |
TTTTGGCAGAGGGAAAAAGA Rabbit beta-globin polyA region, reverse primer |
| CAT-R |
GCAACTGACTGAAATGCCTC 5' end of chloramphenicol resistance gene, reverse primer |
| CMV Forward |
CGCAAATGGGCGGTAGGCGTG (Invitrogen) Human CMV immediate early promoter, forward primer |
| CRE-R |
GCAAACGGACAGAAGCATTT 5' end of Cre recombinase, reverse primer |
| CYC1 |
GCGTGAATGTAAGCGTGAC (Invitrogen) CYC1 transcription termination signal, reverse primer |
| DsRed1-C |
AGCTGGACATCACCTCCCACAACG (BD Biosciences) 3' end of DsRed1, forward primer |
| DsRed1-N |
GTACTGGAACTGGGGGGACAG (BD Biosciences) 5' end of DsRed1, reverse primer |
| EBV Reverse |
GTGGTTTGTCCAAACTCATC (Invitrogen) SV40 polyA terminator, reverse primer |
| Ecdysone Forward |
CTCTGAATACTTTCAACAAGTTAC (Invitrogen) Drosophila heat shock promoter, forward primer |
| EF-1a Forward |
TCAAGCCTCAGACAGTGGTTC (Invitrogen) Human elongation factor-1a promoter, forward primer |
| EGFP-C |
CATGGTCCTGCTGGAGTTCGTG (BD Biosciences) 3' end of EGFP, forward primer |
| EGFP-N |
CGTCGCCGTCCAGCTCGACCAG (BD Biosciences) 5' end of EGFP, reverse primer |
| EXFP-R |
GTCTTGTAGTTGCCGTCGTC (Golenbock lab) For distinguishing EGFP vs ECFP vs EYFP, reverse primer |
| F1ori-F |
GTGGACTCTTGTTCCAAACTGG F1 origin, forward primer |
| GAL1 |
AATATACCTCTATACTTTAACGTC (Invitrogen) S. cerevisiae GAL1 promoter, forward primer |
| Gal10pro-F |
GGTGGTAATGCCATGTAATATG (Stratagene) S. cerevisiae GAL10 promoter, forward primer |
| Gal4 N-term |
GAGTAGTAACAAAGGTCAA 3' end of Gal4 DNA binding domain, forward primer |
| Gal4-AD |
AATACCACTACAATGGAT (BD Biosciences) 3' end of Gal4 activation domain, forward primer |
| GFP-F |
GGTCCTTCTTGAGTTTGTAAC 3' end of GFP, forward primer |
| GFP-R |
CCATCTAATTCAACAAGAATTGGGACAAC (Ahmad lab) 5' end of GFP, reverse primer |
| GPDpro-F |
CGGTAGGTATTGATTGTAATTCTG S. cerevisiae GPD promoter, forward primer |
| GW-3' |
GCATGATGACCACCGATATG 3' end of Gateway cassette, forward primer |
| GW-5' |
AATCTCGCCGGATCCTAACT 5' end of Gateway cassette, reverse primer |
| H1 |
TCGCTATGTGTTCTGGGAAA Human H1 promoter, forward primer |
| HA-F |
TACCCATACGACGTCCCAGA HA tag, forward primer |
| HA-R |
TCTGGGACGTCGTATGGGTA HA tag, reverse primer |
| HAT |
GAGGAGCACGCTCATGCCCAC (BD Biosciences) Histidine affinity tag, forward primer |
| hGH-PA-R |
CCAGCTTGGTTCCCAATAGA Human growth hormone terminator, reverse primer |
| hrGFP-R |
TCCCCGAGTACCACTTCATC hrGFP (humanized Renilla GFP), forward primer |
| hUBCpro-F |
TGAAGCTCCGGTTTTGAACT Human Ubiquitin C (UbC) promoter, forward primer |
| IRES-F |
TGGCTCTCCTCAAGCGTATT 3' end of IRES, forward primer |
| IRES-R |
CCTCACATTGCCAAAAGACG 5' end of IRES, reverse primer |
| L4440 |
AGCGAGTCAGTGAGCGAG (Caldwell lab) 5' of MCS in L4440 vector, forward primer |
| LacI-R |
GGCATACTCTGCGACATCGT 5' end of LacI, reverse primer |
| LacZ-R |
GACAGTATCGGCCTCAGGAA 5' end of LacZ, reverse primer |
| LexA |
CGTCAGCAGAGCTTCACCATTG (Caldwell lab) 3' end of LexA DNA binding domain, forward primer |
| LKO.1 5' |
GACTATCATATGCTTACCGT (Weinberg Lab) Human U6 promoter, forward primer |
| LNCX |
AGCTCGTTTAGTGAACCGTCAGATC (BD Biosciences) Human CMV promoter, forward primer |
| Luc-F |
AGTCAAGTAACAACCGCGA 3' end of luciferase, forward primer |
| LucNrev |
CCTTATGCAGTTGCTCTCC 5' end of luciferase, reverse primer |
| M13 (-21) Forward |
TGTAAAACGACGGCCAGT In lacZ gene |
| M13 (-40) |
GTTTTCCCAGTCACGAC In lacZ gene |
| M13 Reverse |
CAGGAAACAGCTATGAC In lacZ gene |
| M13/pUC Forward |
CCCAGTCACGACGTTGTAAAACG (Invitrogen) In lacZ gene |
| M13/pUC Reverse |
AGCGGATAACAATTTCACACAGG (Invitrogen) In lacZ gene |
| MBP-F |
GATGAAGCCCTGAAAGACGCGCAG (Waugh lab) 3' end of maltose binding protein, forward primer |
| mCherry-F |
CCCCGTAATGCAGAAGAAGA 3' end of mCherry, forward primer |
| mCherry-R |
TTGGTCACCTTCAGCTTGG 5' end of mCherry, reverse primer |
| MT Forward |
CATCTCAGTGCAACTAAA (Invitrogen) Drosophila metallothionein promoter, forward primer |
| MMLV-F |
ATCAGTTCGCTTCTCGCTTC Moloney murine leukemia virus LTR (MoMuLV), forward primer |
| mPGK-F |
CATTCTGCACGCTTCAAAAG Mouse PGK promoter, forward primer |
| MSCV |
CCCTTGAACCTCCTCGTTCGACC (BD Biosciences) Murine stem cell virus, forward primer |
| MSCV-rev |
CAGCGGGGCTGCTAAAGCGCATGC Murine stem cell virus, reverse primer |
| MT1-F |
GCTGTCCTCTAAGCGTCACC Mouse metallothionein 1 promoter, forward primer |
| mU6-F |
CAGCACAAAAGGAAACTCACC Mouse U6 promoter, forward primer |
| Myc |
GCATCAATGCAGAAGCTGATCTCA (BD Biosciences) Myc tag, forward primer |
| Neo-F |
CGTTGGCTACCCGTGATATT 3' end of neomycin resistance gene, forward primer |
| Neo-R |
GCCCAGTCATAGCCGAATAG 5' end of neomycin resistance gene, reverse primer |
| NOS-F |
GCGTTCAAAAGTCGCCTAAG Nopaline synthase promoter, forward primer |
| Nmt1-F |
GCAATGTGCAGCGAAACTAA S. pombe nmt1 promoter, forward primer |
| OpIE2 Forward |
CGCAACGATCTGGTAAACAC (Invitrogen) OpIE2 promoter, forward primer |
| pACYC-F |
TGAAGTCAGCCCCATACGAT p15A origin, forward primer |
| pAd-CMV |
GCTAGAGATCTGGTACCGTC For cloning sites after SalI in pAd-CMV vector |
| pBABE 3' |
ACCCTAACTGACACACATTCC (Weinberg Lab) SV40 enhancer, 3' of MCS in pBABE vectors, reverse primer |
| pBABE 5' |
CTTTATCCAGCCCTCAC (Weinberg Lab) Psi packaging signal, 5' of MCS in pBABE vectors, forward primer |
| pBAD Forward |
ATGCCATAGCATTTTTATCC (Invitrogen) For vectors with E. coli araBAD promoter, forward primer |
| pBAD Reverse |
GATTTAATCTGTATCAGG (Invitrogen) For vectors with E. coli araBAD promoter, reverse primer |
| pBluescriptKS |
TCGAGGTCGACGGTATC For pBluescript vector |
| pBluescriptSK |
TCTAGAACTAGTGGATC For pBluescript vector |
| pBMN 5' |
GCTTGGATACACGCCGC MMLV sequence, for inserts in pBMN retroviral vector |
| pBR322ori-F |
GGGAAACGCCTGGTATCTTT pBRS322 origin, forward primer |
| pBRforBam |
CTTGGAGCCACTATCGAC In pBR322 tet region, upstream of BamHI, forward primer |
| pBRforEco |
AATAGGCGTATCACGAGGC In pBR322, upsteam of EcoRI site, forward primer |
| pBRrevBam |
GGTGATGTCGGCGATATAGG In pBR322 tet region, downstream of BamHI, reverse primer |
| pCAG-F |
GCAACGTGCTGGTTATTGTG Rabbit beta-globin intron, for pCAG plasmids, forward primer |
| pCasper-F |
GGGTTTTATTAACTTACAT (Vosshall lab) 5' end of Drosophila mini-white gene, reverse primer |
| pCasper-hs |
GCAACTACTGAAATCTGCCAAG Drosophila Hsp70 promoter, forward primer |
| pcDL-F |
GTTGCCTTTACTTCTAGGCCT (Kinet lab) 5' of EcoRI site in pcDL vector, forward primer |
| pENTR-F |
CTACAAACTCTTCCTGTTAGTTAG 5' of attL1 in pENTR vector, forward primer |
| pENTR-R |
ATGGCTCATAACACCCCTTG 3' of attL2 in pENTR vector, reverse primer |
| pGEX 3' |
CCGGGAGCTGCATGTGTCAGAGG 3' of MCS in pGEX vectors, reverse primer |
| pGEX 5' |
GGGCTGGCAAGCCACGTTTGGTG 3' end of glutathione-S-transferase, forward primer |
| pGP704-R |
AACAAGCCAGGGATGTAACG R6K gamma origin, 3' of MCS in pGP704 vector, reverse primer |
| pHybLex Reverse |
GAGTCACTTTAAAATTTGTATACAC (Invitrogen) ADH terminator, reverse primer |
| pLTet-F |
ACTGAGCACATCAGCAGGAC Lambda phage early leftward (pL) promoter, forward primer |
| pLXSN 5' |
CCCTTGAACCTCCTCGTTCGACC (MSCV) Murine stem cell virus, same as MSCV, forward primer |
| pMRB101-F |
AAGATGCAGGCAGCTGAGTT HCMV major immediate-early protein (IE), forward primer |
| pMT2-F |
TTGCCTTTCTCTCCACAGGT 3' end of synthetic intron, forward primer |
| pMX-S1811 |
GACGGCATCGCAGCTTGGATACAC (Yamanaka lab) MMLV sequence, 5' of MCS in pMXs vector, forward primer |
| Polyhedrin forward |
AAATGATAACCATCTCGC (Invitrogen) Polyhedrin promoter, forward primer |
| Polyhedrin reverse |
GTCCAAGTTTCCCTG (Invitrogen) For baculovirus vector with polyhedrin promoter, reverse primer |
| pQE promoter |
CCCGAAAAGTGCCACCTG (Qiagen) 5' of MCS in pQE vectors, forward primer |
| pREP Forward |
GCTCGATACAATAAACGCC (Invitrogen) Rous sarcoma virus (RSV) promoter, forward primer |
| pRS-marker |
CGGCATCAGAGCAGATTGTA To sequence yeast selectable marker in pRS vectors |
| Pry1 |
CTTAGCATGTCCGTGGGGTTTGAAT PZ P-element, reverse primer |
| pTrcHis Forward |
GAGGTATATATTAATGTATCG (Invitrogen) 5' of MCS in pTrcHis vector, forward primer |
| pTrcHis Reverse |
GATTTAATCTGTATCAGG (Invitrogen) 3' of MCS in pTrcHis vector, same as pBAD-R, reverse primer |
| Puro-F |
GCAACCTCCCCTTCTACGAGC 3' end of puromycin resistance gene, forward primer |
| pZIP |
TCCTTTCCAGCGAGGTTCTA Murine leukemia virus (MuLV), reverse primer |
| RCAS-F |
ACATGGGTGGTGGTATAGCGCTTGCG (Orsulic lab) 3' of Rous sarcoma virus (RSV) env gene, forward primer |
| Rluc-F |
CCAGGATTCTTTTCCAATGC 3' end of Renilla luciferase, forward primer |
| RVprimer3 |
CTAGCAAAATAGGCTGTCCC (Promega) 5' of MCS in pGL3 vector, forward primer |
| SFFV-F |
ATTGATTGACTGCCCACCTC Spleen focus forming virus 5' LTR, forward primer |
| SP6 |
ATTTAGGTGACACTATAG SP6 promoter, forward primer |
| SV40pA-R |
GAAATTTGTGATGCTATTGC SV40 polyA, reverse primer |
| SV40pro-F |
TATTTATGCAGAGGCCGAGG SV40 promoter/origin, forward primer |
| SV40-spliceR |
CACAAAGATCCGGACCAAAG SV40 splice sequence, reverse primer |
| T3 |
GCAATTAACCCTCACTAAAGG T3 promoter, forward primer |
| T7 |
TAATACGACTCACTATAGGG T7 promoter, forward primer |
| T7 Terminal |
GCTAGTTATTGCTCAGCGG T7 terminator, reverse primer |
| Tac promoter |
GAGCGGATAACAATTTCACACAGG (Waugh lab) Tac promoter, forward primer |
| tdTomato-Fwd |
CTGTTCCTGTACGGCATGG 3' end of tdTomato, forward primer |
| tdTomato-Rev |
TCTTTGATGACGGCCATGT 5' end of tdTomato, reverse primer |
| Tet-R |
GGCGAGTTTACGGGTTGTTA 5' end of tetracycline resistance gene, reverse primer |
| TK-pA-R |
TTGTCTCCTTCCGTGTTTCA Thymidine kinase polyA, reverse primer |
| Tn7-end |
GGGGTGGAAATGGAGTTTTT Bacterial transposon Tn7 |
| TRC-F |
CAAGGCTGTTAGAGAGATAATTGGA (Root lab) Human U6 promoter, forward primer |
| Ubx-F |
AACTCGTACTTTGAACAGGC Drosophila Ultrabithorax gene, forward primer |
| V5 Reverse |
ACCGAGGAGAGGGTTAGGGAT (Invitrogen) V5 epitope, reverse primer |
| WPRE-R |
CATAGCGTAAAAGGAGCAACA 5' end of WPRE, reverse primer |
| XBG-R |
GACTCCATTCGGGTGTTC Xenopus beta-globin 3'UTR, reverse primer |
| XEF1a |
TTTCGCCCTAACTTCGTGAT Xenopus EF1 alpha enhancer/promoter, forward primer |
| Xpress Forward |
TATGGCTAGCATGACTGGT (Invitrogen) Xpress epitope, forward primer |
